@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAkfBGQhLswDRvhfS6ezvS5_Jme5hF5Ii-0dOeBVezgiI> .
@prefix sub: <http://purl.org/np/RAkfBGQhLswDRvhfS6ezvS5_Jme5hF5Ii-0dOeBVezgiI#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  sub:_1 rdfs:label "pellet" .
  
  sub:step dce:description "After centrifuging at 5400 g at 4˚C for 5 min, remove the supernatant and then air-dry the pellet.";
    a pplan:Step;
    bs:bioSampleUsed sub:_1 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-13T15:32:59.311324"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:KP_nxHbbEeuq3QNGPvIb7Q, authority:UTm0_nbbEeuuGcMK-6f5Kg .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "PUXlgDeM1HgMP7A89ZMO85gsCIskj6Fg/RxodD10mV0i9b93Fb8COv0ra6k9tOKOghojKmrBCySpLvB8rjH00f8DnTRcDG8oer65yGqxyaUpJcSVXZrTL3isgQWn98ENu35feUl7zE8bqX9NX2I5P8UC9d+rBlDLRJKvaARkUmU=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-13T15:32:59.311324"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}