@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAlgTxpazWzhn8HnguDf-L50Hp71CaUErFDZ5iCMjxulM> .
@prefix sub: <http://purl.org/np/RAlgTxpazWzhn8HnguDf-L50Hp71CaUErFDZ5iCMjxulM#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/CHEBI_16236> rdfs:label "70% RNase-free ethanol" .
  
  sp:RNAPellet rdfs:label "RNA pellet" .
  
  sub:step dce:description "RNA pellet was washed with 1 ml 70% RNase-free ethanol once and spin down by 7,500 x g for 5 min.";
    sp:hasExperimentalOutput sp:RNAPellet;
    a pplan:Step;
    bs:bioSampleUsed sp:RNAPellet;
    bs:reagentUsed <http://purl.obolibrary.org/obo/CHEBI_16236> .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-05T15:58:00.648553"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:ARGFan3yEeuctFtfjRECmg, authority:FqMVGn3yEeuk4Gvjmr_Lnw,
      authority:OABAwH3yEeuVWxeojFDwvQ .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "NVF+pEx/G15Zw6t8aIODreWPNB2mDMeToVt0olQCyP6EfMxfUvIPyAX+paNuBRAmq5/Nbs4GCW0FG+QsfGicxGHWsyjLxoVxOI0huvVoZcQKgwyT+DRiSWvn/RUS0FDC64QuaSdT/Hyd2Fi7X2tpuvCgLb9O62zUGO91e9EGnWo=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-05T15:58:00.648553"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}