@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAmfmsVve1Fpi5N8Ilwa8I2iwfA2voEOMFafY5gLtSEOY> .
@prefix sub: <http://purl.org/np/RAmfmsVve1Fpi5N8Ilwa8I2iwfA2voEOMFafY5gLtSEOY#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  sub:_1 rdfs:label "upper aqueous phase" .
  
  sub:step dce:description "Centrifuge suspension at 12,000 x g for 10 min, 4 °C. Carefully transfer upper aqueous phase to a new 50 ml tube.";
    a pplan:Step;
    bs:bioSampleUsed sub:_1, <http://www.bioassayontology.org/bao#BAO_0002104>;
    bs:labEquipmentUsed <http://www.bioassayontology.org/bao#BAO_0010014> .
  
  <http://www.bioassayontology.org/bao#BAO_0002104> rdfs:label "suspension" .
  
  <http://www.bioassayontology.org/bao#BAO_0010014> rdfs:label "50 ml tube" .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-20T05:42:13.116235"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:URp5AolmEeu4PWvmYljShA, authority:ZqN5IolmEeujev-H_9cUXw,
      authority:fK7lWIlmEeuJ2oOJ1YFPxg, authority:lVvDyIlmEeuPp2-9XFIY4Q .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "YfEl5jIEJPyeCRFUi38eWI6yn3SUuQblns6KE61cUzWZwP0ro/GH3cx2RRbnkvIdPWLf8QGl14/gg/g0+bErZtpzi9SibgLpjhEbPdB7DIvK65w4VEKAonkdkzxQOITSQ+Ux52zn1ksLHauGCJ2tcC1CDclXTyOd4aiEPO3GhdI=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-20T05:42:13.116235"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}