@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAoFLU3ESOZSGO1Rr-yAal9NoRvoFQUHHQtiF2zi5DsJE> .
@prefix sub: <http://purl.org/np/RAoFLU3ESOZSGO1Rr-yAal9NoRvoFQUHHQtiF2zi5DsJE#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/OBI_0000918> rdfs:label " Dulbecco's modified eagle medium." .
  
  <http://purl.obolibrary.org/obo/UBERON_0000178> rdfs:label " blood " .
  
  sub:_1 rdfs:label "cells" .
  
  sub:_2 rdfs:label "cells" .
  
  sub:_3 rdfs:label "PBS" .
  
  sub:step dce:description "Wash (centrifuge in 100 x g for 10 min) twice with 10 ml of sterile PBS or sterile Dulbecco's modified eagle medium. The approximate yield of cells from 4 ml of blood varies between 107-108.";
    sp:hasExperimentalInput <http://purl.obolibrary.org/obo/UBERON_0000178>;
    sp:hasExperimentalOutput sub:_1;
    a pplan:Step;
    bs:bioSampleUsed <http://purl.obolibrary.org/obo/UBERON_0000178>, sub:_2;
    bs:reagentUsed <http://purl.obolibrary.org/obo/OBI_0000918>, sub:_3 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-02-12T03:14:10.508670"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:6C2wGFbsEeuwB8fWwikNrw, authority:J18VxFbtEeuQt5_D40Tv0g,
      authority:QAf0klbtEeupAJcFbklL7A, authority:XmlbklbtEeueshdaAFeLag, authority:qQAHKFckEeuklmcvqgKzZA .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "Ju8BcSYhMdfQj12kdfCCffFIL/G1w1qsWbfNXuvsPUJbcPhOZJ6KTAI6HOiwIr91xiD9/2+AUfnBOPnaaGgMEUIvpJUflpkihVgaw7EodSAXhjAZKn4l12dHnhoc12aqVaxCc2CxFyRps+ouAa5An+tT2RI+89FjjpVEcKWxYm4=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-02-12T03:14:10.508670"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}