@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RApoqP4lv5AOvQUPlRE1i04QMOITLFL3tQuc6kDT-ZBBI> .
@prefix sub: <http://purl.org/np/RApoqP4lv5AOvQUPlRE1i04QMOITLFL3tQuc6kDT-ZBBI#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/OBI_0002488> rdfs:label "1 ml auto pipette" .
  
  <http://purl.obolibrary.org/obo/UBERON_0000178> rdfs:label "blood" .
  
  sub:_1 rdfs:label "Ficoll Histopaque" .
  
  sub:step dce:description "Gently layer the blood on the top of Ficoll Histopaque using a 1 ml auto pipette. The layering should be done very slowly that blood and Ficoll Histopaque should stay as two different layers.";
    sp:hasExperimentalInput <http://purl.obolibrary.org/obo/UBERON_0000178>;
    a pplan:Step;
    bs:bioSampleUsed <http://purl.obolibrary.org/obo/UBERON_0000178>;
    bs:labEquipmentUsed <http://purl.obolibrary.org/obo/OBI_0002488>;
    bs:reagentUsed sub:_1 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-04T15:11:14.099346"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:SoEpUH0jEeu4hOdUUmy_Fg, authority:X1d_-n0jEeuz7d8rRLryUg,
      authority:_N0uzn0iEeuQqqNXdaCtXg, authority:ck26An0jEeuIb3dRiLmm0A .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "Sb47s30On9qBTTKZt7/k76cSg1Q1Y/sgBKsQ5gWjN4GOxh27e1eCfWYInndcGdHierLG57hsT9Zntd6KN5kmGrGtCxMvwVGsFoQEtypFXhw5qnVyGaQLPXN69MZiFyDp/CKqn1PTbF7U4esd+/K3Yjg7vlsfqSX9loPgYSme94k=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-04T15:11:14.099346"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}