@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RApqOsQhrRYLG4NW1jfLFnKdDkz1odV9wuKKLIt2e0hds> .
@prefix sub: <http://purl.org/np/RApqOsQhrRYLG4NW1jfLFnKdDkz1odV9wuKKLIt2e0hds#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/ERO_0000979> rdfs:label "freezer" .
  
  sub:_1 rdfs:label "tubes" .
  
  sub:_2 rdfs:label "95% ethanol" .
  
  sub:step dce:description "Add 2 volumes of cold 95% ethanol and mix gently by inversion. The tubes are incubated at for 45 min in -20°C freezer. It should not be for more time as some remaining phenolics and resin may also precipitate with DNA.";
    a pplan:Step;
    bs:labEquipmentUsed <http://purl.obolibrary.org/obo/ERO_0000979>, sub:_1;
    bs:reagentUsed sub:_2 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-10T10:49:12.235538"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:3K_LLoFMEeuXme8vxx8oQg, authority:8kymtIFMEeu79-u8LzCp6Q,
      authority:DyUXMIFNEeuydwPNhy_SGg, authority:xj0RgIFMEeuHfaunX377mg .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "ZLsTWMW+HZIA0Dibt3lLNr6vDd+f4wPRnSiYIeFfBPzGjmxG2Qh7D9OoxbRH+BjBjfpL9JdbvKb6ZDWtElMIkwqTDwBu7pnGtfhPUhVFoXsnhgz8V6rmmIw8YaOIggvWCLsX4eiup9BcyZYg1CntPUcKcdcS+wBEWOTqUvjcgLM=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-10T10:49:12.235538"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}