@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RApqru-5znOZx4R0ojWOzXbto00lxwgRjiT7eDFr4Hkuo> .
@prefix sub: <http://purl.org/np/RApqru-5znOZx4R0ojWOzXbto00lxwgRjiT7eDFr4Hkuo#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/CHEBI_26710> rdfs:label "NaCl" .
  
  sub:_1 rdfs:label "supernatant" .
  
  sub:_2 rdfs:label "RNAse A" .
  
  sub:_3 rdfs:label "chloroform: isoamyl alcohol" .
  
  sub:step dce:description "After RNAse A treatment, add one volume of chloroform: isoamyl alcohol again and mix by inversions for 5 min. The tube is centrifuged for 10 min at 5000 × g at 4°C. Transfer the clear supernatant into new 2ml tube and add half volume of 5 M NaCl to the sample and mix gently by inversions.";
    a pplan:Step;
    bs:bioSampleUsed sub:_1;
    bs:labEquipmentUsed <http://www.bioassayontology.org/bao#BAO_0010014>;
    bs:reagentUsed <http://purl.obolibrary.org/obo/CHEBI_26710>, sub:_2, sub:_3 .
  
  <http://www.bioassayontology.org/bao#BAO_0010014> rdfs:label "2ml tube", "tube" .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-02-25T16:22:29.532808"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:5Y6WEHetEeuHODMQ_uavRg, authority:UWlV4netEeuPjz-MC5DRcQ,
      authority:ZDicFHetEeujJZMQKn2jcA, authority:dxW3QHetEeuk4qdeznt6Kg, authority:jh_usHetEeuphLPMcTw5HA,
      authority:sA79_netEeuXTIsjwlxzrw, authority:w1ErinetEeu2sDfL5YgtOg .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "IVSwQ7ovexd+K3TlSVaIpqeLtDsotw2p09er2G/DY/fMMJOsdhfuH2CzMX6PmVEfjeX2W2WaxconGjG/nkBEyCyEuuR/TUcpQ5L19tiuwybOlc+ml2c+4eIkVq4WlszLmYp6KG/sjp7nvBb7ZCHA0ReKxwnEM940AP0rKy6QkZY=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-02-25T16:22:29.532808"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}