@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAqgGDA2OoEZap-M5MFyuOBVNA8dk4sJ9YwzuaaWzv47c> .
@prefix sub: <http://purl.org/np/RAqgGDA2OoEZap-M5MFyuOBVNA8dk4sJ9YwzuaaWzv47c#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  sub:_1 rdfs:label "mycobacteria" .
  
  sub:_2 rdfs:label "mycobacteria" .
  
  sub:_3 rdfs:label "mortar" .
  
  sub:_4 rdfs:label "TE buffer" .
  
  sub:step dce:description "Add 10 ml TE buffer into the mortar and carefully bring mycobacteria in suspension. Transfer this suspension to a 50 ml polypropylene tube.";
    sp:hasExperimentalInput sub:_1;
    a pplan:Step;
    bs:bioSampleUsed sub:_2;
    bs:labEquipmentUsed sub:_3, <http://www.bioassayontology.org/bao#BAO_0010014>;
    bs:reagentUsed sub:_4 .
  
  <http://www.bioassayontology.org/bao#BAO_0010014> rdfs:label "50 ml polypropylene tube." .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-22T16:13:19.826780"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:F-dBWng8Eeuhr2Ohj2Be5Q, authority:KAm1Xng8Eeuoj_P45YQy_Q,
      authority:TZhp0ng8EeuOmrv-P6nU4w, authority:eFAHVHezEeu6Kzd3vep0zQ, authority:ejkQ9Hg8Eeu8QsMJ1t57XQ .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "K+C6Rinudf53OkKOYIHH1NeJdoAiNjExHryAMpmOPj4qgiAaompwzUvjxI7F6GslHo7iLa5//rZ+Ve3hA2SyjjXgrnWafq+nv0G34/zWN8by6LNbQzPA1vzW4gfa6V7q1mIYsdWmrnGrlETcput8+UclmHk2bPyERF4Z/x57DZg=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-22T16:13:19.826780"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/zuluaga.lizzz> .
}