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Fig. 3. Transcriptional induction of gene 33 in response to vasoactive peptides, calcium ionophore, and mechanical strain: Induction by mechanical strain requires SAPK activity. A, rat renal mesangial cells were treated with endothelin (ET-1), angiotensin-II (A-II), or calcium ionophore (A23187) as indicated (see \"Experimental Procedures\"). gene 33 induction was determined by Northern blot. gapdh expression served as a loading control. B, pulmonary A549 cells were infected with the indicated recombinant adenoviruses at the indicated Pfu/cell and, after 48 h, subjected to mechanical strain (see \"Experimental Procedures\"). gene 33 induction was determined by Northern blot. gapdh expression served as a loading control.
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