@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAs4beaaVqUBJUjFT8azzLj76uG8bpnQbpM5PsXIxBkB4> .
@prefix sub: <http://purl.org/np/RAs4beaaVqUBJUjFT8azzLj76uG8bpnQbpM5PsXIxBkB4#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  <http://purl.obolibrary.org/obo/CHEBI_16991> rdfs:label "DNA" .
  
  <http://purl.obolibrary.org/obo/CHEBI_17824> rdfs:label "isopropanol" .
  
  <http://purl.obolibrary.org/obo/CHEBI_33697> rdfs:label "RNA" .
  
  sub:_1 rdfs:label "tubes" .
  
  sub:step dce:description "Add 700 µl of cold isopropanol to precipitate RNA or DNA and then invert tubes 3-4 times to mix the solution.";
    sp:hasExperimentalOutput <http://purl.obolibrary.org/obo/CHEBI_16991>, <http://purl.obolibrary.org/obo/CHEBI_33697>;
    a pplan:Step;
    bs:bioSampleUsed <http://purl.obolibrary.org/obo/CHEBI_16991>, <http://purl.obolibrary.org/obo/CHEBI_33697>;
    bs:labEquipmentUsed sub:_1;
    bs:reagentUsed <http://purl.obolibrary.org/obo/CHEBI_17824> .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-03-05T11:38:14.803520"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:5h3XAH3IEeu8UYsrJ6SWMQ, authority:boIdUH3IEeu-XoshKyI_Vw,
      authority:f6yVVn3IEeuz2M9MGndh8A, authority:nK_LRn3IEeuHt_N31BtMdQ, authority:sVFiWH3IEeunh3tsgtSR_Q .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "GTnhmHFvXL5orLWoDQy7vIXv8nTzGCDlvreGc5IRaufB7lkr6UdO4yyMcABosngeez8vqUo7TZJ+IY58CVMoDbGXMpXdVMXVZUqp1Bo+/67tr1E1qAtJ5gmo10CymNJ7c9TcuCE3TnZrrxDLsCzjt4ZYEI3PoNTxCSPD5n9Zibc=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-03-05T11:38:14.803520"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/iannotate> .
}