@prefix dce: <http://purl.org/dc/elements/1.1/> .
@prefix pplan: <http://purl.org/net/p-plan#> .
@prefix this: <http://purl.org/np/RAtBK5K6kf9TGKBImruq2EL1E-OKUOww92e5wcyGNDo_U> .
@prefix sub: <http://purl.org/np/RAtBK5K6kf9TGKBImruq2EL1E-OKUOww92e5wcyGNDo_U#> .
@prefix bs: <https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#> .
@prefix np: <http://www.nanopub.org/nschema#> .
@prefix pav: <http://purl.org/pav/> .
@prefix authority: <https://hypothes.is/a/> .
@prefix npx: <http://purl.org/nanopub/x/> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix prov: <http://www.w3.org/ns/prov#> .
@prefix sp: <http://purl.org/net/SMARTprotocol#> .

sub:Head {
  this: np:hasAssertion sub:assertion;
    np:hasProvenance sub:provenance;
    np:hasPublicationInfo sub:pubInfo;
    a np:Nanopublication .
}

sub:assertion {
  sub:_1 rdfs:label "RNA pellets" .
  
  sub:_2 rdfs:label "RNA pellets" .
  
  sub:_3 rdfs:label "RNase-free H2O" .
  
  sub:step dce:description "The RNA pellets are air-dried and dissolved in 100 µl RNase-free H2O by incubating at 65 °C for 15 min";
    sp:hasExperimentalOutput sub:_1;
    a pplan:Step;
    bs:bioSampleUsed sub:_2;
    bs:reagentUsed sub:_3 .
}

sub:provenance {
  sub:assertion pav:createdWith <https://nanotate.bitsfetch.com>;
    prov:generatedAtTime "2021-02-18T10:01:08.001697"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}

sub:pubInfo {
  sub:assertion prov:wasDerivedFrom authority:DzkCKnH6Eeuvu9cxICeKxw, authority:Qo-ddHFAEeuJvTe6gqIIPA,
      authority:XvNzKHFAEeuhY1NRz0oV0g .
  
  sub:sig npx:hasAlgorithm "RSA";
    npx:hasPublicKey "MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB";
    npx:hasSignature "YM5OJ0PKYEuWputpbkWNvmXmyDeV5cOpppvV9IKPAujJBSbY905agOMnH+UjzHFqLzDMiz2JDY6F92RlLOHPO0BYIfH/Qg8tWtSg47zHZPkabRd3q8G5kDF4+2KgShaBXv76Br0kEUjhy+9c2Ndq3RumzNq8g99SubbpZu0OX1c=";
    npx:hasSignatureTarget this: .
  
  this: npx:introduces sub:step;
    prov:generatedAtTime "2021-02-18T10:01:08.001697"^^xsd:dateTime;
    prov:wasAttributedTo <https://hypothes.is/users/alejandra.sandoval> .
}