http://www.tkuhn.ch/bel2nanopub/RAtFxvCrYaOP3Ss2iChmHLHNT2qdRuc8QBAf2TCfrIcN8#Head http://www.tkuhn.ch/bel2nanopub/RAtFxvCrYaOP3Ss2iChmHLHNT2qdRuc8QBAf2TCfrIcN8 http://www.nanopub.org/nschema#hasAssertion http://www.tkuhn.ch/bel2nanopub/RAtFxvCrYaOP3Ss2iChmHLHNT2qdRuc8QBAf2TCfrIcN8#assertion http://www.tkuhn.ch/bel2nanopub/RAtFxvCrYaOP3Ss2iChmHLHNT2qdRuc8QBAf2TCfrIcN8 http://www.nanopub.org/nschema#hasProvenance http://www.tkuhn.ch/bel2nanopub/RAtFxvCrYaOP3Ss2iChmHLHNT2qdRuc8QBAf2TCfrIcN8#provenance http://www.tkuhn.ch/bel2nanopub/RAtFxvCrYaOP3Ss2iChmHLHNT2qdRuc8QBAf2TCfrIcN8 http://www.nanopub.org/nschema#hasPublicationInfo http://www.tkuhn.ch/bel2nanopub/RAtFxvCrYaOP3Ss2iChmHLHNT2qdRuc8QBAf2TCfrIcN8#pubinfo http://www.tkuhn.ch/bel2nanopub/RAtFxvCrYaOP3Ss2iChmHLHNT2qdRuc8QBAf2TCfrIcN8 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.nanopub.org/nschema#Nanopublication http://www.tkuhn.ch/bel2nanopub/RAtFxvCrYaOP3Ss2iChmHLHNT2qdRuc8QBAf2TCfrIcN8#assertion http://www.tkuhn.ch/bel2nanopub/RAtFxvCrYaOP3Ss2iChmHLHNT2qdRuc8QBAf2TCfrIcN8#_1 http://purl.obolibrary.org/obo/BFO_0000066 http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=9606 http://www.tkuhn.ch/bel2nanopub/RAtFxvCrYaOP3Ss2iChmHLHNT2qdRuc8QBAf2TCfrIcN8#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#object http://purl.bioontology.org/ontology/MSH/D017209 http://www.tkuhn.ch/bel2nanopub/RAtFxvCrYaOP3Ss2iChmHLHNT2qdRuc8QBAf2TCfrIcN8#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate http://www.selventa.com/vocabulary/increases http://www.tkuhn.ch/bel2nanopub/RAtFxvCrYaOP3Ss2iChmHLHNT2qdRuc8QBAf2TCfrIcN8#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#subject http://www.ebi.ac.uk/chebi/searchId.do?chebiId=8897 http://www.tkuhn.ch/bel2nanopub/RAtFxvCrYaOP3Ss2iChmHLHNT2qdRuc8QBAf2TCfrIcN8#_1 http://www.w3.org/1999/02/22-rdf-syntax-ns#type http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement http://www.tkuhn.ch/bel2nanopub/RAtFxvCrYaOP3Ss2iChmHLHNT2qdRuc8QBAf2TCfrIcN8#assertion http://www.w3.org/2000/01/rdf-schema#label a(CHEBI:rotenone) -> bp(MESHPP:Apoptosis) http://www.tkuhn.ch/bel2nanopub/RAtFxvCrYaOP3Ss2iChmHLHNT2qdRuc8QBAf2TCfrIcN8#provenance http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/description Approximately 61,000 statements. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/rights Copyright (c) 2011-2012, Selventa. All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAtFxvCrYaOP3Ss2iChmHLHNT2qdRuc8QBAf2TCfrIcN8#_3 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RAtFxvCrYaOP3Ss2iChmHLHNT2qdRuc8QBAf2TCfrIcN8#_2 http://www.w3.org/ns/prov#value The degeneration of nigral dopamine neurons in Parkinson's disease (PD) reportedly involves a defect in brain mitochondrial complex I in association with the activation of nuclear factor-kappaB (NF-kappaB) and caspase-3. To elucidate molecular mechanisms possibly linking these events, as well as to evaluate the neuroprotective potential of the cyclopentenone prostaglandin A1 (PGA1), an inducer of heat shock proteins (HSPs), we exposed human dopaminergic SH-SY5Y cells to the complex I inhibitor rotenone. Dose-dependent apoptosis was preceded by the nuclear translocation of NF-kappaB and then the activation of caspase-3 over the ensuing 24 h. PGA1 increased the expression of HSP70 and HSP27 and protected against rotenone-induced apoptosis, without increasing necrotic death. PGA1 blocked the rotenone-induced nuclear translocation of NF-kappaB and attenuated, but did not abolish, the caspase-3 elevation. Unexpectedly, the caspase-3 inhibitor, Ac-DEVD.CHO (DEVD), at a concentration that completely prevented the caspase-3 elevation produced by rotenone, failed to protect against apoptosis. These results suggest that complex I deficiency in dopamine cells can induce apoptosis by a process involving early NF-kappaB nuclear translocation and caspase-3 activation. PGA1 appears to protect against rotenone-induced cell death by inducing HSPs and blocking nuclear translocation of NF-kappaB in a process that attenuates caspase-3 activation, but is not mediated by its inhibition. http://www.tkuhn.ch/bel2nanopub/RAtFxvCrYaOP3Ss2iChmHLHNT2qdRuc8QBAf2TCfrIcN8#_2 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/12437580 http://www.tkuhn.ch/bel2nanopub/RAtFxvCrYaOP3Ss2iChmHLHNT2qdRuc8QBAf2TCfrIcN8#_3 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAtFxvCrYaOP3Ss2iChmHLHNT2qdRuc8QBAf2TCfrIcN8#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/12437580 http://www.tkuhn.ch/bel2nanopub/RAtFxvCrYaOP3Ss2iChmHLHNT2qdRuc8QBAf2TCfrIcN8#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAtFxvCrYaOP3Ss2iChmHLHNT2qdRuc8QBAf2TCfrIcN8#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAtFxvCrYaOP3Ss2iChmHLHNT2qdRuc8QBAf2TCfrIcN8#_2 http://www.tkuhn.ch/bel2nanopub/RAtFxvCrYaOP3Ss2iChmHLHNT2qdRuc8QBAf2TCfrIcN8#pubinfo http://www.tkuhn.ch/bel2nanopub/RAtFxvCrYaOP3Ss2iChmHLHNT2qdRuc8QBAf2TCfrIcN8 http://purl.org/dc/terms/created 2014-07-03T14:30:07.292+02:00 http://www.tkuhn.ch/bel2nanopub/RAtFxvCrYaOP3Ss2iChmHLHNT2qdRuc8QBAf2TCfrIcN8 http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAtFxvCrYaOP3Ss2iChmHLHNT2qdRuc8QBAf2TCfrIcN8 http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234