[ { "@graph" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAulIv-rYSNHgKNWGZEFcS27ryN5ae4cZPrDAlCXRdmp4", "@type" : [ "http://www.nanopub.org/nschema#Nanopublication" ], "http://www.nanopub.org/nschema#hasAssertion" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAulIv-rYSNHgKNWGZEFcS27ryN5ae4cZPrDAlCXRdmp4#assertion" } ], "http://www.nanopub.org/nschema#hasProvenance" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAulIv-rYSNHgKNWGZEFcS27ryN5ae4cZPrDAlCXRdmp4#provenance" } ], "http://www.nanopub.org/nschema#hasPublicationInfo" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAulIv-rYSNHgKNWGZEFcS27ryN5ae4cZPrDAlCXRdmp4#pubinfo" } ] } ], "@id" : "http://www.tkuhn.ch/bel2nanopub/RAulIv-rYSNHgKNWGZEFcS27ryN5ae4cZPrDAlCXRdmp4#Head" }, { "@graph" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAulIv-rYSNHgKNWGZEFcS27ryN5ae4cZPrDAlCXRdmp4#_1", "@type" : [ "http://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI_36080" ], "http://purl.obolibrary.org/obo/RO_0002204" : [ { "@id" : "http://www.informatics.jax.org/marker/MGI:88388" } ] }, { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAulIv-rYSNHgKNWGZEFcS27ryN5ae4cZPrDAlCXRdmp4#_2", "http://www.selventa.com/vocabulary/translocationFrom" : [ { "@id" : "http://amigo.geneontology.org/amigo/term/GO:0005622" } ], "http://www.selventa.com/vocabulary/translocationOf" : [ { "@id" : "http://resource.belframework.org/belframework/1.0/namespace/selventa-legacy-chemical-names/Chloride%281-%29" } ], "http://www.selventa.com/vocabulary/translocationTo" : [ { "@id" : "http://amigo.geneontology.org/amigo/term/GO:0005576" } ] }, { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAulIv-rYSNHgKNWGZEFcS27ryN5ae4cZPrDAlCXRdmp4#_3", "@type" : [ "http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement" ], "http://www.w3.org/1999/02/22-rdf-syntax-ns#object" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAulIv-rYSNHgKNWGZEFcS27ryN5ae4cZPrDAlCXRdmp4#_2" } ], "http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate" : [ { "@id" : "http://www.selventa.com/vocabulary/increases" } ], "http://www.w3.org/1999/02/22-rdf-syntax-ns#subject" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAulIv-rYSNHgKNWGZEFcS27ryN5ae4cZPrDAlCXRdmp4#_1" } ] }, { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAulIv-rYSNHgKNWGZEFcS27ryN5ae4cZPrDAlCXRdmp4#_4", "@type" : [ "http://www.w3.org/1999/02/22-rdf-syntax-ns#Statement" ], "http://purl.obolibrary.org/obo/BFO_0000066" : [ { "@id" : "http://purl.bioontology.org/ontology/MSH/D004848" }, { "@id" : "http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=10090" } ], "http://www.w3.org/1999/02/22-rdf-syntax-ns#object" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAulIv-rYSNHgKNWGZEFcS27ryN5ae4cZPrDAlCXRdmp4#_3" } ], "http://www.w3.org/1999/02/22-rdf-syntax-ns#predicate" : [ { "@id" : "http://www.selventa.com/vocabulary/increases" } ], "http://www.w3.org/1999/02/22-rdf-syntax-ns#subject" : [ { "@id" : "http://resource.belframework.org/belframework/1.0/namespace/selventa-legacy-chemical-names/cyclic%20AMP" } ] }, { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAulIv-rYSNHgKNWGZEFcS27ryN5ae4cZPrDAlCXRdmp4#assertion", "http://www.w3.org/2000/01/rdf-schema#label" : [ { "@value" : "a(SCHEM:\"cyclic AMP\") -> (p(MGI:Cftr) -> sec(a(SCHEM:\"Chloride(1-)\")))" }, { "@value" : "p(MGI:Cftr) -> sec(a(SCHEM:\"Chloride(1-)\"))" } ] } ], "@id" : "http://www.tkuhn.ch/bel2nanopub/RAulIv-rYSNHgKNWGZEFcS27ryN5ae4cZPrDAlCXRdmp4#assertion" }, { "@graph" : [ { "@id" : "http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel", "http://purl.org/dc/elements/1.1/description" : [ { "@value" : "Approximately 61,000 statements." } ], "http://purl.org/dc/elements/1.1/rights" : [ { "@value" : "Copyright (c) 2011-2012, Selventa. All rights reserved." } ], "http://purl.org/dc/elements/1.1/title" : [ { "@value" : "BEL Framework Large Corpus Document" } ], "http://purl.org/pav/authoredBy" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAulIv-rYSNHgKNWGZEFcS27ryN5ae4cZPrDAlCXRdmp4#_6" } ], "http://purl.org/pav/version" : [ { "@value" : "1.4" } ] }, { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAulIv-rYSNHgKNWGZEFcS27ryN5ae4cZPrDAlCXRdmp4#_5", "http://www.w3.org/ns/prov#value" : [ { "@value" : "We investigated adenosine (Ado) activation of the cystic fibrosis transmembrane conductance regulator (CFTR) in vitro and in vivo. A(2B) Ado receptors were identified in Calu-3, IB-3-1, COS-7, and primary human airway cells. Ado elevated cAMP in Calu-3, IB-3-1, and COS-7 cells and activated protein kinase A-dependent halide efflux in Calu-3 cells. Ado promoted arachidonic acid release from Calu-3 cells, and phospholipase A(2) (PLA(2)) inhibition blocked Ado-activated halide efflux in Calu-3 and COS-7 cells expressing CFTR. Forskolin- and beta(2)-adrenergic receptor-stimulated efflux were not affected by the same treatment. Cytoplasmic PLA(2) (cPLA(2)) was identified in Calu-3, IB-3-1, and COS-7 cells, but cPLA(2) inhibition did not affect Ado-stimulated cAMP concentrations. In cftr(+) and cftr(-/-) mice, Ado stimulated nasal Cl(-) secretion that was CFTR dependent and sensitive to A(2) receptor and PLA(2) blockade. In COS-7 cells transiently expressing DeltaF508 CFTR, Ado activated halide efflux. Ado also activated G551D CFTR-dependent halide efflux when combined with arachidonic acid and phosphodiesterase inhibition. In conclusion, PLA(2) and protein kinase A both contribute to A(2) receptor activation of CFTR, and components of this signaling pathway can augment wild-type and mutant CFTR activity." } ], "http://www.w3.org/ns/prov#wasQuotedFrom" : [ { "@id" : "http://www.ncbi.nlm.nih.gov/pubmed/11741811" } ] }, { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAulIv-rYSNHgKNWGZEFcS27ryN5ae4cZPrDAlCXRdmp4#_6", "http://www.w3.org/2000/01/rdf-schema#label" : [ { "@value" : "Selventa" } ] }, { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAulIv-rYSNHgKNWGZEFcS27ryN5ae4cZPrDAlCXRdmp4#assertion", "http://www.w3.org/ns/prov#hadPrimarySource" : [ { "@id" : "http://www.ncbi.nlm.nih.gov/pubmed/11741811" } ], "http://www.w3.org/ns/prov#wasDerivedFrom" : [ { "@id" : "http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel" }, { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAulIv-rYSNHgKNWGZEFcS27ryN5ae4cZPrDAlCXRdmp4#_5" } ] } ], "@id" : "http://www.tkuhn.ch/bel2nanopub/RAulIv-rYSNHgKNWGZEFcS27ryN5ae4cZPrDAlCXRdmp4#provenance" }, { "@graph" : [ { "@id" : "http://www.tkuhn.ch/bel2nanopub/RAulIv-rYSNHgKNWGZEFcS27ryN5ae4cZPrDAlCXRdmp4", "http://purl.org/dc/terms/created" : [ { "@type" : "http://www.w3.org/2001/XMLSchema#dateTime", "@value" : "2014-07-03T14:29:59.531+02:00" } ], "http://purl.org/pav/createdBy" : [ { "@id" : "http://orcid.org/0000-0001-6818-334X" }, { "@id" : "http://orcid.org/0000-0002-1267-0234" } ] } ], "@id" : "http://www.tkuhn.ch/bel2nanopub/RAulIv-rYSNHgKNWGZEFcS27ryN5ae4cZPrDAlCXRdmp4#pubinfo" } ]