@prefix pplan: <
http://purl.org/net/p-plan#
> .
@prefix this: <
http://purl.org/np/RAxls8cPUy9udLdFmYKHAl3COavnUDq74RzCgDGO1z2QQ
> .
@prefix sub: <
http://purl.org/np/RAxls8cPUy9udLdFmYKHAl3COavnUDq74RzCgDGO1z2QQ#
> .
@prefix bs: <
https://bioschemas.org/profiles/LabProtocol/0.4-DRAFT-2020_07_23/#
> .
@prefix np: <
http://www.nanopub.org/nschema#
> .
@prefix pav: <
http://purl.org/pav/
> .
@prefix authority: <
https://hypothes.is/a/
> .
@prefix npx: <
http://purl.org/nanopub/x/
> .
@prefix xsd: <
http://www.w3.org/2001/XMLSchema#
> .
@prefix rdfs: <
http://www.w3.org/2000/01/rdf-schema#
> .
@prefix prov: <
http://www.w3.org/ns/prov#
> .
@prefix dc: <
http://purl.org/dc/elements/1.1/
> .
sub:Head
{
this:
np:hasAssertion
sub:assertion
;
np:hasProvenance
sub:provenance
;
np:hasPublicationInfo
sub:pubInfo
;
a
np:Nanopublication
.
}
sub:assertion
{
sub:_1
rdfs:label
"suspension" .
sub:_2
rdfs:label
"aqueous phase" .
sub:_3
rdfs:label
"50 ml tube" .
sub:step
dc:description
"Centrifuge suspension at 12,000 x g for 10 min, 4 °C. Carefully transfer upper aqueous phase to a new 50 ml tube" ;
a
pplan:Step
;
bs:bioSampleUsed
sub:_1
,
sub:_2
;
bs:labEquipmentUsed
sub:_3
.
}
sub:provenance
{
sub:assertion
pav:createdWith
<
https://nanotate.bitsfetch.com
> ;
prov:generatedAtTime
"2021-03-22T16:25:19.318973"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/alejandra.sandoval
> .
}
sub:pubInfo
{
sub:assertion
prov:wasDerivedFrom
authority:3vdroIFUEeuapg9M7S4Uvw
,
authority:9c4hSIFUEeuewlv2AF8Enw
,
authority:B-WeEIFVEeuCtPMAsO4KVw
,
authority:GvHj4oFVEeuWVzcA447L-A
.
sub:sig
npx:hasAlgorithm
"RSA" ;
npx:hasPublicKey
"MIGfMA0GCSqGSIb3DQEBAQUAA4GNADCBiQKBgQCEOtYac15zsWN/LAu3q7YFdW0UZNfX3BAarHW/LB1DHGcz0yYr0v7hKGbnCLJ+UkDfu3Mgj7BnCm1s8ulMULKfF4YjqS2YKHYZjNKVaIjSigFOpjOwIx99+qPlvd5CuG+Gia/sJA05jy2bPKLwb/kmVeeX0zORZudWLyRMLXp8UQIDAQAB" ;
npx:hasSignature
"YSR3gB/XLHrvLiR8AKqzFdq4HxmipifeTGP0CEV99lLCghfgveD+YUj6zRPFm8FrO4qlonKdaIuUmmolnqqR+S/DtEZu3XTz5BgwfF1TCoDOEkjq6Kj1gY5UKU/ws9rD4t90lhZvq8Adi3AlWq11f50OK+GzLUDD4PDfw5c58Zw=" ;
npx:hasSignatureTarget
this:
.
this:
npx:introduces
sub:step
;
prov:generatedAtTime
"2021-03-22T16:25:19.318973"^^
xsd:dateTime
;
prov:wasAttributedTo
<
https://hypothes.is/users/alejandra.sandoval
> .
}