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All rights reserved. http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/dc/elements/1.1/title BEL Framework Large Corpus Document http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/authoredBy http://www.tkuhn.ch/bel2nanopub/RAybSKc4mks8eLPD7A8YCZ5QUuXLt0wr0-pSVKKvlyjjA#_8 http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://purl.org/pav/version 1.4 http://www.tkuhn.ch/bel2nanopub/RAybSKc4mks8eLPD7A8YCZ5QUuXLt0wr0-pSVKKvlyjjA#_7 http://www.w3.org/ns/prov#value Next, we sought to analyse whether the decrease in FADD-FLICE association caused by PED overexpression affected FLICE protease activity. To this end, we incubated identical amounts of in vitro translated 35S-PARP, a death substrate cleaved by the active FLICE, with extracts from control or PED-overexpressing cells stimulated with IgMFAS. A major band, corresponding to the 26 kDa cleavage product of 35S-PARP was identified after incubation with extracts from IgMFAS-stimulated control cells (Figure 8). The intensity of the band was fivefold lower after PARP incubation with extracts from FAS-unstimulated cells. Most important, in this assay, PARP cleavage was threefold less effective in PED overexpressing than in control cells. No 26 kDa band was detectable in the absence of cell extract addition. Thus, cleavage of death substrates by active FLICE occurs less efficiently in cells overexpressing PED. http://www.tkuhn.ch/bel2nanopub/RAybSKc4mks8eLPD7A8YCZ5QUuXLt0wr0-pSVKKvlyjjA#_7 http://www.w3.org/ns/prov#wasQuotedFrom http://www.ncbi.nlm.nih.gov/pubmed/10442631 http://www.tkuhn.ch/bel2nanopub/RAybSKc4mks8eLPD7A8YCZ5QUuXLt0wr0-pSVKKvlyjjA#_8 http://www.w3.org/2000/01/rdf-schema#label Selventa http://www.tkuhn.ch/bel2nanopub/RAybSKc4mks8eLPD7A8YCZ5QUuXLt0wr0-pSVKKvlyjjA#assertion http://www.w3.org/ns/prov#hadPrimarySource http://www.ncbi.nlm.nih.gov/pubmed/10442631 http://www.tkuhn.ch/bel2nanopub/RAybSKc4mks8eLPD7A8YCZ5QUuXLt0wr0-pSVKKvlyjjA#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://resource.belframework.org/belframework/1.0/knowledge/large_corpus.bel http://www.tkuhn.ch/bel2nanopub/RAybSKc4mks8eLPD7A8YCZ5QUuXLt0wr0-pSVKKvlyjjA#assertion http://www.w3.org/ns/prov#wasDerivedFrom http://www.tkuhn.ch/bel2nanopub/RAybSKc4mks8eLPD7A8YCZ5QUuXLt0wr0-pSVKKvlyjjA#_7 http://www.tkuhn.ch/bel2nanopub/RAybSKc4mks8eLPD7A8YCZ5QUuXLt0wr0-pSVKKvlyjjA#pubinfo http://www.tkuhn.ch/bel2nanopub/RAybSKc4mks8eLPD7A8YCZ5QUuXLt0wr0-pSVKKvlyjjA http://purl.org/dc/terms/created 2014-07-03T14:29:47.998+02:00 http://www.tkuhn.ch/bel2nanopub/RAybSKc4mks8eLPD7A8YCZ5QUuXLt0wr0-pSVKKvlyjjA http://purl.org/pav/createdBy http://orcid.org/0000-0001-6818-334X http://www.tkuhn.ch/bel2nanopub/RAybSKc4mks8eLPD7A8YCZ5QUuXLt0wr0-pSVKKvlyjjA http://purl.org/pav/createdBy http://orcid.org/0000-0002-1267-0234