. . . . . . . . . . . . . . . . . "kin(p(HGNC:MAPK1)) -> gtp(p(HGNC:GNAI3))" . "Approximately 61,000 statements." . "Copyright (c) 2011-2012, Selventa. All rights reserved." . "BEL Framework Large Corpus Document" . . "1.4" . "# Ariadne: An in vitro phosphorylation assay demonstrates that Erk2-dependent phosphorylation of GAIP stimulates its GTPase-activating protein activity toward the Galpha i3 protein ( k = 0.187 .. 0.001 s -1 , EC 50 = 1.12 .. 0.10 .. M ) when compared with unphosphorylated recombinant GAIP ( k = 0.145 .. 0.003 s -1 , EC 50 = 3.16 .. 0.12 .. M ) or to GAIP phosphorylated by other Ser/Thr protein kinases (protein kinase C, casein kinase II). [Regulation] # Ariadne: Using a panel of Ser/Thr protein kinases in an in vitro assay, we demonstrate that the phosphorylation of GAIP by a recombinant Erk2 stimulated its GAP activity toward the Galpha i3 protein when compared with the activity of unphosphorylated GAIP . [Regulation]" . . "Selventa" . . . . "2014-07-03T14:29:52.407+02:00"^^ . . .